Topic: Immunity status of the human body. CP 1.

⇐ ПредыдущаяСтр 17 из 24Следующая ⇒

Date__________________

CLASS 5

Topic: Immunity status of the human body. CP 1.

Questions to be discussed:

1. Immunity status of the body, definition, stages of evaluation.

2. 1^st and 2^nd level tests of the immunity status evaluation.

Practical tasks:

Fill in a table with the tests used for immunity status evaluation.

Immune status

tests

1^st level tests

2nd level tests

Control point 1.

I. Practical tasks: draw

- surface receptors (TLR, TCR, BCR, MHC-1/2), or immunoglobulin molecule, indicate important fragments (domains) and their functions;

- cells (APC, NK, Т- / В-lymphocytes, indicate important functional receptors, CD markers, secreted cytokines and interleukins, their function;

- schemes of complement activation, type-1 interferon action, phagocytosis, NK-mediated killing, MHC class 1/2 restricted antigen recognition, endogenous and exogenous antigen processing, cellular and mediator cooperation in humoral and cellular immune response development, effector functions of the antibodies, indicate important functional receptors, CD markers, cytokines;

- mechanisms of the diagnostic reactions (agglutination, precipitation, complement fixation, virus and toxins neutralization, heamagglutination inhibition, ELISA, IF, immunoblotting.

II. Oral answer

Questions for oral answer:

1. Immunity. Types and forms of immunity. Characteristic of innate and acquired defense mechanisms.

2. Factors of Innate immunity, their characteristic features.

3. Inflammation as a protective reaction against infection. Stages of inflammation.

4. Pathogen-associated molecular patterns (PAMPs) – “non-self’, and damage associated molecular patterns (DAMPs) – “Damaged self”, their characteristic, examples.

5. Pattern recognition receptors (PRR), their categories (surface, endosomal, soluble).

6. Phagocytosis. Phagocytes. Stages of phagocytosis. Mechanism of killing. Complete and incomplete phagocytosis.

7. NK cells, characteristic, mechanism of target recognition and killing.

8. Complement system, its components. Classical, alternative and lectin activation pathways. Biological effects of complement.

9. Interferons, their classification and functions. Mechanism of anti-viral activity. The way of production. Application.

10. Cells of the immune system: immunocompetent, antigen-presenting, antigen-nonspecific.

11. Antigens, their characteristic and main properties. Complete and incomplete antigens. Bacterial and viral antigens. Group-specific, species-specific, and type-specific antigens of microorganisms.

12. Human antigens. Genetic organization of the Major Histocompatibility complex (MHC). Class I and class II MHC antigens, structure and function. Significance in immune response development.

13. Cluster of differentiation – CD molecules, their identification. Human leukocyte markers.

14. B-lymphocytes: stages of differentiation, characteristic features, functions. BCR, structure and function.

15. T lymphocytes: populations and subpopulations of T lymphocytes, characteristic and function. Surface markers and receptors.

16. T lymphocytes: structure and function of TCR. Differences in a function of α /β and γ /δ TCR types.

17. Characteristic of Th1 and Th2 lymphocytes. CD markers, interleukins, function. Regulatory T lymphocytes (Treg), their characteristic, surface markers, function.

18. Exogenous antigen processing and presentation. Endogenous antigen processing and presentation.

19. Antibody (immunoglobulins). Classes of immunoglobulins, their differences in structure and function.

20. Humoral immune response development. Cooperation of antigen-presenting cells, T and B cells by receptors and cytokines (mediators). T-dependent and T-independent immune responses.

21. Primary and secondary immune responses. Immunological memory.

22. Serological agglutination tests: direct, indirect (passive), main components. Modifications of agglutination test. Application.

23. Serological precipitation tests. Characteristic of main components. Ring and gel precipitation, immunoelectrophoresis. Application.

24. Cells involved in generation of cell-mediated immune responses: main surface receptors, function, lymphokines and monokines.

25. Receptor and humoral cell-cooperation in cell-mediated immune response.

26. Effector functions of T cytotoxic lymphocytes.

27. Effector functions of Th1 DH lymphocytes.

28. Mechanism of anti-tumor immunity development.

29. Immunity in transplantation.

30. Characteristic features of anti-bacterial immunity.

31. Characteristic features of antiviral immunity.

32. Immune status of the human organism. Methods of immune response estimation.

33. Complement fixation test, reagents and mechanism.

34. Serological reactions used for virus identification and anti-viral immunity evaluation (Nt test, HI test).

35. Immunological reactions with labeled reagents, their advantages. IF, ELISA, RIA.

36. Virus neutralizing tests. Practical use.

37. Hemagglutination inhibition test. Practical use.

Teacher’s signature ________________

⇐ Предыдущая 12 13 14 15 161718 19 20 21 Следующая ⇒