Culture and identification has to be done in reference laboratories with biosafety level 1-2 (RF) or 3-4 (USA) only

Date___________

Cass 16

Topic: Causative agents of biohazadous diseases: variola virus, Ebola and Marburg hemorrhagic fever. Microorganisms as a potential warfare agents. Basic principles of the diagnosis and prevention.

Questions to be discussed:

General features of the biohazadours diseases.
Microorganisms as potential biological warfare agents.
Criteria for the agents that can be used as biological weapon.
Poxviridae: variola virus, morphology, structure, and culture.
Pathogenesis and clinical manifestation of the smallpox. Eradication of smallpox.
Filoviridae: Ebola and Marburg viruses.
Basic principles of prevention.

Practical tasks:

Study the basic principles of the laboratory diagnosis of biohazadous diseases:

- rapid indication: determination of the antigens or genes of the agent in a specimen that takes less than 3 hours; (IF, ELISA, PCR); - rapid identification: determination of the antigens or genes of the agent in a specimen within 3 hours; (tests are specific for particular agent); - traditional methods of diagnostics for complete identification of the species and types: culture; - serology: determination of the Ab in blood.

Study the main structural elements of the variola virus:

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Study a scheme of variola virus replication, label the main stages of replication:

4. List methods used for variola virus culture and methods of its detection: _________________________________________________________________________________________________________________________________________________________________________________________________________

Describe vaccine against smallpox:

Label the main structural elements of the Ebola virus:

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Label the main stages of Ebola virus replication:

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8. List methods used for Ebola virus culture and methods of its detection: _____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

Complete a scheme of Ebola and Marburg viruses hemorrhagic fever diagnosis:

Specimen:
Rapid diagnosis		Serology
IF ELISA (for specific antigens)		ELISA for antibody
PCR (for viral RNAs)
Culture and identification has to be done in reference laboratories with biosafety level 1-2 (RF) or 3-4 (USA) only

Describe vaccine against Ebola virus:

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Teacher’s signature ________________

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