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Togaviridae. Flaviviridae.  Bunyaviridae. __method



Togaviridae

    

Flaviviridae

   

 Bunyaviridae

 

7. Complete a scheme of the flavivirus replicative cycle and label its main stages:

 

8. Indicate the approaches used for some arboviruses culturing and methods of their detection:

Family Togaviridae: ___________________________________________________________________________
_____________________________________________________________________________

Family Flaviviridae: ______________________________________________________________________________________________________________________________________________

Family Bunyaviridae: _____________________________________________________________________________________________________________________________________________

 

9. Complete a scheme of the laboratory diagnosis of tick-born encephalitis:

 

Specimen:                
______method

__________________method

________method ____________method
Determine specific Ag: IF, ELISA, passive HA test   Determine viral NA: PCR   1. Culturing:              Primary and diploid cell cultures  
 


Chicken embryo

  Suckling mice inoculation
 

 

 


Death

Determination of antibody titer increase in paired blood sera: HI, CFT          Nt test                          Ig M    ELISA                                     Ig G
  2. Indication: CPE, HAds test   HA test     Nt test             
 

3. Viral identification:

 

HI assay CFT Nt test ELISA                    

   

 

 

10. Characterize immuno-biological preparations used for diagnosis, prophylaxis and treatment of arboviral fevers and encephalitis: (immunoglobulins, vaccines, interferon, diagnostic immune sera and antigens):

Specific for tick-borne viral encephalitis immunoglobulins: ______________

_____________________________________________________________________

 

Tick-born viral encephalitis vaccine: __________________________________

_____________________________________________________________________

 

Yellow fever vaccine: ________________________________________

_____________________________________________________________________

 

Rubella vaccine: ___________________________________________________

_____________________________________________________________________

 

 

Teacher’s signature ________________


 



  

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