Laboratory phase

We observed that masilla and mixtures of masilla and bagasse are good sources of nutrients for mycelial growth on solid cultures and good production of fruiting bodies in both P. ostreatus and P. pulmonarius. For the first time we used masilla, bagasse and a combination of the two (1: 1) to observe mycelial growth of Pleurotus spp. The strains of Pleurotus spp (IBUG-4 and IBUG-8) grew better in masilla substrates than in bagasse alone.

Mycelial growth rate

Mycelial growth of P. ostreatus (IBUG-8) and P. pulmonarius (IBUG-4) was observed on all masilla media, and we detected a linear growth promotion effect with increasing concentrations of masilla (Figure 1). The effect was much stronger for P. pulmonarius (IBUG-4) than for P. ostreatus (IBUG-8) as expressed by the linear regression coefficients. This was true for growth rate, but not for the biomass production response variable. The growth rate of IBUG-4 was from 3. 2 to 3. 9 mm/day in 10% and 50% masilla, and 2. 8 mm/day in MEA. For IBUG-8 the growth rate was from 3. 2 to 3. 9 mm/day in 30 and 50% masilla, respectively, and 3. 7 mm/day in MEA.

Figure 1. Effect of beer masilla concentration on growth rate of Pleurotus ostreatus (IBUG-8)

and P. pulmonarius (IBUG-4).

Biomass production

Biomass production of P. ostreatus (IBUG-8) and P. pulmonarius (IBUG-4)strains on MEA and masilla is shown in Table 1. Biomass production for P. pulmonarius was from 164. 4 to 164. 6 mg/dish in 50% and 10% of beer masilla and 160. 33 mg/dish in MEA. In P. ostreatus, biomass production was from 161. 6±6 mg/dish to 163. 2±93 mg/dish in 50% and 30% masilla, and 170. 2 mg/dish in MEA. Masilla allowed a greater growth rate and biomass production than other tequila industry wastes, such as tequila vinasses (Madrigal et al. 2000) and bitter honey (Arias and Fausto 1999).

Table 1. Biomass production of Pleurotus ostreatus (IBUG-8) and P. pulmonarius (IBUG-4) on

MEA and beer masilla media.

Strain	Culture media	Biomass production (mg/dish)
IBUG- 4	10*	164. 6 ± 26
		164. 5 ± 55
		164. 4 ± 37
	MEA	160. 3 ± 20
IBUG- 8		161. 7 ± 25
		163. 2 ± 56
		161. 6 ± 34
	MEA	170. 2 ± 19

*= % beer masilla

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