Mushroon strains. Culture media. Conditions for mycelial growth in laboratory phase

Mushroon strains

A strain of Pleurotus ostreatus (IBUG-8) and another of P. pulmonarius (IBUG-4) were used. They are deposited in the mycological culture collection of the Department of Botany and Zoology, University of Guadalajara, Jalisco, Mexico.

Culture media

Beer masilla media

Spent brewer's grain was obtained from the “Cervecerí a Modelo” brewery in Guadalajara, Jalisco. The masilla was filtered and diluted to 10, 30, and 50% with distilled water. Malt extract agar (MEA) was used as a control medium. Culture media were autoclaved at 121 °C for 15 min and 20 ml of each placed in petri dishes 90 mm in diameter. The pH of the media was 4. 0 before sterilization.

Substrate media

The filtrated beer masilla was mixed with maguey tequila bagasse for qualitative mycelial growth of Pleurotus strains. Three petri dishes per strain and three mixtures of masilla: bagasse substrates (0: 1, 1: 1 and 1: 0) were used.

Conditions for mycelial growth in laboratory phase

Inocula preparation

For preparation of inocula, the fungus was grown on MEA plates at 28 °C for 7 days. Mycelium agar plugs 6 mm in diameter, cut along the edge of an actively growing colony, were used as inocula.

Inoculation

Five plates per media and strain containing 20 ml of medium and a cellophane membrane, were inoculated with a mycelium agar plug, and maintained at 28 °C for 11 days.

Mycelial growth rate

Mycelial growth rate was determined (mm/day) by measuring the diameter of culture growth in the petri dish on days 3 to 11.

Biomass production

Fungal biomass was quantified by gravimetry on dry mass at 80°C up to constant weight (mg/dish) after 11 days of incubation.

Spawn preparation

Spawn was grown on wheat grains in high density polyethylene bags. The bags were sterilized 45 minutes at 121°C and inoculated with P. ostreatus (IBUG-8) or P. floridanus (IBUG-4) (Soto-Velazco et al. 1993).

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