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Topic: Herpesviruses. Laboratory diagnosis of herpesvirus’ infections.

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Date_____________

Class 13

Topic: Herpesviruses. Laboratory diagnosis of herpesvirus’ infections.

ISW: Prions. Prion infections of animals and humans.

Questions to be discussed:

  1. Classification of Family Herpesviridae.
  2. Morphology and structure of the herpesviruses..
  3. Replicative cycle of herpesviruses.
  4. Alpha-herpesviruse subfamily: characteristic features, tissue tropism, and diseases.
  5. Beta-herpesviruse subfamily: characteristic features, tissue tropism, and diseases.
  6. Gamma-herpesviruse subfamily: characteristic features, tissue tropism, and diseases.
  7. Lymphoproliferative, oncogenic and embiotoxic activity of the herpesviruses.
  8. Immunity in herpesviruses’ infections and persistancy of viruses.
  9. Laboratory diagnosis of alpha-, beta-, and gamma-herpesviruses’ infections.
  10. Ant-herpesvirus chemotherapy. Immunobiological preparations used in herpesviruses’ infections.

Practical tasks:

  1. Draw and label the main structural components of the herpesvirus virion.
___________________________________ ___________________________________ ___________________________________ ___________________________________ ___________________________________ ___________________________________ ____________________________________

 

  1. List the main stages of herpesvirus replication and label targets for the antivirals.
     __________________________________________________________________________________________________________________________________________________________________________________________________________________ ____________________________________

    

  1.  Indicate CPE effects produced by subfamilies:

 Alphaherpesvirinae:

 ___________________________________________________________

______________________________________________________________________________________________________________________________________________

 Betaherpesvirinae: ­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

 ­­­­­­___________________________________________________________ ­­­­­­_____________________________________________________________________________________________________________________________________________

Gammaherpesvirinae:

________________________________________________________ _______________________________________________________________________________________________________________________________________________________

  1. Complete a scheme of the laboratory diagnosis of genital herpes diagnosis.

 

Specimen:    
 

 

 
________ ____________ ______________ _____________
  1. Virus isolation:  Cells: Human embryo fibroblasts, chicken embryo AB identification in patient’s paired sera:
Detection of multinucleated (Tzanck) cells   Direct IF (virus identification) _________method   2. Virus detection:       CFT ELISA
    PCR Identification of viral DNA   3. Virus identification: ___________________ __________________-____________________  
  1. Describe the mechanism of EBV infection.

  1. Describe the action of anti-herpes drugs.

SW: Prions. Prion infections of animals and humans.

1. Properties and stricture of prions.

2. Prion infections of animals (skrepi, mad caw disease) and humans (kuru, Creitsfield-Jakobs).

3. Laboratory diagnosis of prion infections.

 

Teacher’s signature ________________

 


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