Topic: Pathogenic Spirochetes: Treponema, Borrelia, Leptospira. Laboratory diagnosis of syphilis, borrelioses, leptospiroses.

Treponemal tests:

Non-treponemal tests:

FTA-Abs

______________________________________

______________________________________

______________________________________

MHA-TP ______________________________________ ______________________________________

^2st serum

ELISA ______________________________________ _____________________________________

VDRL (flocculation) test

PCR __________________________________

RPR test

_______________________________________

Data__________________

Class 5

Topic: Pathogenic Spirochetes: Treponema, Borrelia, Leptospira. Laboratory diagnosis of syphilis, borrelioses, leptospiroses.

ISW: Rickettsiae that cause epidemic and endemic typhus. Laboratory diagnosis of rickettsial diseases.

Questions to be discussed:

1. Distinguishing features of Spirochetes structure.

2. Pathogenicity factors of Treponema pallidum – causative agent of syphilis.

3. Pathogenesis of syphilis. Stages of the disease. Congenital syphilis.

4. Laboratory diagnosis of syphilis on the different stages of the disease.

5. Wassermann’s complement fixation test.

6. Causative agents of nonveneral trepanematoses.

7. Distinguishing features of Borrelia causing epidemic and endemic relapsing fever.

8. Pathogenesis of relapsing fever.

9. Characteristic of Borrelia burgdorferi, which causes tick-borne Lyme disease.

10. Laboratory diagnosis of borrelioses.

11. Characteristic of Leptospira.

12. Pathogenic factors of L. intrerrhogans. Pathogenesis of leptospiroses.

13. Laboratory diagnosis of leptospiroses.

Practical tasks:

Examine microscopically and draw hard chancre exudate, stained by collargol negative staining.

2. . List virulence properties of Тreponema pallidum: ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

Read and draw the result of Wasserman test.

Complete a scheme of the lab diagnoses of syphilis:

Specimen: _______________________________________________________________________

Microscopy:

______________ _______________ ________________ Тreponema pallidum in hard chancre exudates, stained by collargol negative staining.

5. List virulence factors of Borrelia: ______________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

6. Complete a scheme of laboratory diagnosis of borrelioses:

Specimen: ____________________________________________________

Microscopy		Animal inoculation	Serology
______________	________________
Staining		After 5 days – blood examination	Indirect IF test
			ELISA
PCR
		Staining by_______

7. List virulence factors of Leptospira intrerrhogans: ______________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

Complete a scheme of the laboratory diagnoses of leptospiroses:

Specimen: _______________________________________________________

Microscopy

Culture

Animal inoculation

PCR

t=28º -30º C in СО₂

______________

____________

Medium: ____________

_________________

After 10 days of culture

t=28º -30º C in СО₂ after 7-10 days of culturing

Serology

Identification:

Agglutination – lyses test

Biochemical properties

37º С - 1 hour

Patient’s serum + alive Leptospira

Antigenic structure

Describe preparations used for diagnoses of syphilis, borrelioses, and leptospiroses: cardiolipin, lyophilized complement, borreliae antigens, leptospiral diagnostic sera.

Describe preparations used for specific prophylaxis and treatment of leptospiroses: vaccine, anti-leptospiral immunoglobulns:

Anti-leptospiral immunoglobuln: ____________________________________________________

_______________________________________________________________________________

______________________________________________________________________________

Leptospiral vaccine: ______________________________________________________________

______________________________________________________________________________________________________________________________________________________________

Teacher’s signature ___________________

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