4. Complete and describe the scheme of culture and identification of meningococcal infections

4. Complete and describe the scheme of culture and identification of meningococcal infections

Culture: blood or CSF is cultured on chocolate and blood agar in an atmosphere containing 5% of CO₂ at 37⁰C for 48 hours. Oxidase test is performed on colonies on solid medium. Sugar utilization test or commercial kits are used to identify meningococci.

Specimen:

1 stage

Staining by ________________

Inoculation on enriched selective medium or serum agar

2 stage

Oxidase test

3 stage

Subculture in differential media for biochemical properties

Culture identification based on sugar fermentation and serological identification of the meningococcal capsule antigen by IF, ELISA and serovars

Non-culturable methods:

- Capsule polysaccharide demonstration in CSF;

- Latex-agglutination test;

- PCR-based techniques.

List pathogenic factors of:

Meningococci Gonococci

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7. Describe preparations that can be used for treatment, diagnosis and prophylaxis of gonococcal and meningococcal infections: (antibiotics, vaccines, diagnostic meningococcal sera, ELISA test-systems).

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Table 1.

Identification of the Neisseria species

Species	Growth on BA, 22 0C	СО2 requirements	Yellow pigment	Sugar fermentation
Species	Growth on BA, 22 0C	СО2 requirements	Yellow pigment	Glucose	Maltose	Sucrose
N. gonorroeae	-	+	-	+	-	-
N. meningitidis	-	+	-	+	+	-
N. flava	+	-	+	-	-	-
N. mucosa	+	-	+/-	+	+	+

Teacher’s signature _________________

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