Topic: Retroviruses. HIV viruses. Laboratory diagnosis of the HIV infection and AIDS.

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Date____________

Class 14

Topic: Retroviruses. HIV viruses. Laboratory diagnosis of the HIV infection and AIDS.

ISW: Oncogenic viruses and infections caused by oncogenic viruses.

Questions to be discussed:

1. Characteristic features of the Family Retroviridae.

2. Morphology and structure of the HIV virion.

3. Reproduction of the HIV, variability of HIV.

4. Pathogenesis of the HIV infection and clynical symptoms of the AIDS.

5. Laboratory diagnosis of the HIV infection and AIDS.

6. Antiviral drugs used for anti-retriviral therapy and their mechanisms of action.

7. Prevention of the HIV infection.

Practical tasks:

1. Дabel the main structural elements of the HIV-1:

2. Complete a scheme of the HIV-1 reproduction and list it’s basic stages:

3. Study time course of HIV infection

4. Describe ELISA, IF, IB test-systems used for the diagnosis of HIV-infection:

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5. Describe the possible application of the reverse PCR in diagnosis of HIV infections and in controlling of the effectiveness of the anti-retroviral therapy.

6. Complete a scheme of the laboratory diagnosis of HIV-infection:

Specimen:
_____________method	_________________method
ELISA for Ab of HIV-1/HIV-2 IFfor Ab of HIV-1/HIV-2	PCR (identification of the provirus DNA in a T-lymphocyte genome)
IB (Ab towards specific HIV Аgs)	Reverse PCR (identification of the viral RNA in patients blood serum)

7. Study characteristic of the main groups of anti-retroviral drugs according to their targets. Give examples of the drugs from each group:

-The anti-HIV drugs target HIV enzymes:

(1) nucleoside analogue reverse transcriptase inhibitors -

(2) nonnucleoside reverse transcriptase inhibitors -

(3) protease inhibitors -

(4) Integrase inhibitors -

-Fusion inhibitors (FIs):

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