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Topic: The Gram-negative cocci pathogenic for humans. Laboratory diagnosis of gonoсcal, and meningococcal infections.

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Date__________________

Class 1

Topic: The Gram-negative cocci pathogenic for humans. Laboratory diagnosis of gonoсcal, and meningococcal infections.

ISW: Haemophilus influenza. Virulence factors. Laboratory diagnosis of Н. influenzae infection.

Questions to be discussed:

1. Morphology and tinctorial properties of gonococci and meningococci.

2. Gonococcal and meningococcal virulence factors.

3. Meningococcal antigenic structure.

4. Outcome of gonococcal and meningococcal infections.

5. Diagnosis of acute and chronic gonococcal infections.

6. Diagnosis of acute meningococcal infections.

7. Immunity after gonococcal and meningococcal infections.

8. Prevention and therapy of gonococcal and meningococcal infections.

Practical tasks:

1. Complete and describe the scheme of culture and identification  of gonococcal infections: Genital, rectal, and pharyngeal specimens must be inoculated on enriched selective media (modified Thayer-Martin medium (MTM) or serum agar and incubated in an atmosphere containing 5% of CO2 at 370C for 48 hours. Identify oxidase-positive activity

Specimen      

1st stage

         
 
   
Inoculation on enriched selective medium or serum agar

 


Staining by _____________________________________

 

2nd stage                                       Oxidase and                 Catalase tests                          
3rd stage       read sugar fermentation patterns and compare it with those in a table -immunofluorescent test with labeled Ab, DNA-probes can be used for confirmation.       

2. Non-culturable methods used in diagnosis of  gonorrhea:

- PCR-based techniques;

- DNA-probes;

 


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