Individual work. student of _____ group. Lesson 7. BIOLOGICAL OXIDATION. THE RESPIRATORY CHAIN. Structure and FUNCTION of DEHYDROGENASES. OXIDATIVE PHOSPHORYLATION. MICROSOMAL CYTOCHROME P450 MONOOXYGENASE SYSTEM.

Individual work

student of _____ group

_______________________________________________

^(name)

Lesson 7. BIOLOGICAL OXIDATION. THE RESPIRATORY CHAIN. Structure and FUNCTION of DEHYDROGENASES. OXIDATIVE PHOSPHORYLATION. MICROSOMAL CYTOCHROME P450 MONOOXYGENASE SYSTEM.

1. Give the definitions of the followings terms:

Biological oxidation – ____________________________________________________________ ____________________________________________________________________________________________________________________________________________________________

Electrochemical potential – ________________________________________________________

____________________________________________________________________________________________________________________________________________________________

2. Indicate a place of localization of a biological oxidation enzymes.

А. External membrane of a mitochondrion;

B. Intermembranous space;

C. Inner membrane of a mitochondrion;

D. Matrix.

3. Name the enzymes of respiratory chain in order of their participation in the electrons and protons transport (S - substrate).

4. Write the oxidated and reducted forms of a coenzyme in composition of the primary dehydrogenases:

5. Write an oxidated and reducted forms of prosthetic group in composition of the secondary dehydrogenases:

6. What is a power of transmission of electrons at a chain of enzymes of a biological oxidation? ___________________________________________________________________________

___________________________________________________________________________

7. What is created on an inner membrane of a mitochondrion as a result of transmission of electrons on a chain of enzymes of a biological oxidation? ____________________________________

____________________________________________________________________________

8. The process of synthesis ATP is catalyzed by enzyme __________________________________

10. Haw, according of the theory of P. Mitchell, is the elecrochemichal potential realized? ____________________________________________________________________________

__________________________________________________________________________________________________________________________________________________________

11. What quantity of protons is transferred from a matrix of a mitochondrion to intermembranous space for one stage of a biological oxidation (transmission on a chain 2 electrons)? __________

12. The final electron acceptor in biological oxidation is: __.

13. What quantity of ATP molecules is synthesized, if the electrons and protons enter the process of a biological oxidation:

а) from primary dehydrogenases ____________________________________________

b) from secondary dehydrogenases ____________________________________________

14. Write the formula of ATP.

15. Give the definition:

Microsomal oxidation – ________________________________________________________

____________________________________________________________________________________________________________________________________________________________

16. In what tissues the monooxygenase systems are localized? ________________________________________________________________________________________________________________________________________________________

________________________________________________________________________________________________________________________________________________________

17. Name the enzymes which are in a composition monooxygenase systems.

1. __________________________________________________________________________

2. __________________________________________________________________________

3. __________________________________________________________________________

4. __________________________________________________________________________

18. Name the substrates of monooxygenase and dioxygenase systems.

1. __________________________________________________________________________

2. __________________________________________________________________________

3. __________________________________________________________________________

4. __________________________________________________________________________

5. __________________________________________________________________________

6. __________________________________________________________________________

19. What is the role of cytochrome Р₄₅₀ in microsomal oxidation?

________________________________________________________________________________________________________________________________________________________

20. Explain a biological role of monooxygenase microsomal systems.

__________________________________________________________________________________________________________________________________________________________________________________________________________________________________________

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