Methods of enzymes investigation

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Lesson 3

Methods of enzymes investigation

1. The primary structure of protein can be determined with the help of:

А. gel-filtration;

B. ultracentrifugation;

C. dispersion of optical rotation;

D. electrophoresis;

E. determination of sequence of nucleotides in the respective genes.

2. Isoelectric point of a peptide asn-leu-trp:

А. is higher than рН 7.0;

B. is in the range рН 7.0;

C. is lower then рН 7.0;

D. depends from рН of solution.

3. The conformation of protein can be determined with the help of:

А. gel-filtration;

B. dispersion of optical rotation;

C. ultracentrifugation;

D. determination of primary structure;

E. electrophoresis.

4. The solubility of proteins is provided, among other, by:

А. high molecular mass;

B. presence of leu, ala and phe residues;

C. presence of arg, tre and asp residues.

5. By what method the secondary structure of protein can be studied?

А. Nuclear magnetic resonance (NMR);

B. Dialysis;

C. Gel-filtration;

D. Ultracentrifugation;

E. Electrophoresis.

Lesson 3

Methods of enzymes investigation

1. Isoelectric point of a peptide gly-val-asn:

A. is higher than рH 7.0;

B. is in range of рН 7.0;

C. is lower than рН 7.0;

D. depends from рН of solution.

2. From what factors the existence of amino acids in equilibrium state (aqueous phase) dose depend?

A. From pH of medium;

B. From temperature of medium;

C. From pK of amino acids;

D. From a charge of amino acids.

3. The conformation of protein can be determined with the help of:

A. gel-filtration;

B. ultracentrifugation;

C. dispersion of optical rotation;

D. determination of primary structure;

E. electrophoresis.

4. What technique is known as "finger printing of proteins or peptide mapping"?

A. Protein digestion by trypsin, followed by two-dimensional chromatography (electroforesis);

B. Chromatography;

C. Aamino acid sequence;

D. Dialysis;

E. Ultracentrifugation.

5. What reaction can be used for qualitative identification and quantitative estimation of proteins?

A. Xanthoprotein test;

B. Ninhydrin reaction;

C. Reaction with copper sulfate;

D. Biuret reaction;

E. Reaction with ammonium sulfate.

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