Methods of enzymes investigation

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Lesson 3

Methods of enzymes investigation

1. At a gel-filtration the first proteins leaving column have:

А. lower molecular weight;

B. high molecular weight;

C. positive charge;

D. negative charge;

E. none of the above.

2. What pH is the best for separation of the mixture of glycine, arginine and aspartate by electrophoresis?

А. 3.0;

B. 7.0;

C. 14.0;

D. 1.0;

E. It is impossible to do by electrophoresis.

3. What method from the following can be used for determination of three-dimension structure of protein molecules?

А. Gel filtration;

B. Ultracentrifugation;

C. X-ray crystallography;

D. Determination of primary structure;

E. Electrophoresis.

4. The normal level of protein in blood serum consists:

А. 6–10 g/l;

B. 16–20 g/l;

C. 30–50 g/l;

D. 65–85 g/l;

E. 100–150 g/l.

5. What proteins are soluble in a half-saturated solution of ammonium sulfate?

А. Globulins;

B. Albumins;

C. Both types of proteins;

D. Any of indicated types of proteins.

Lesson 3

Methods of enzymes investigation

1. Primary structure of any protein may be determined by:

А. X-ray crystallography

B. amino acid sequence

C. dialysis

D. gel-filtration

E. ultracentrifugation

2. At this value of pH the number of anions and cations present on the protein molecule is equal and the net charge is zero. How this value of pH is called?

А. Iso-electric point of a protein

B. Iso-ionic point of a protein

C. Optimum pH

D. Critical pH

E. Limited pH

3. The determination of protein conformation is possible by:

А. electrophoresis;

B. ultracentrifugation;

C. X-ray crystallography;

D. amino acid analysis;

E. dialysis.

4. What proteins will be precipitated by half-saturation of ammonium sulfate?

А. Globulins;

B. Albumins;

C. Globulins and albumins;

D. Neither will be precipitated.

5. What reaction can be used for qualitative identification and quantitative estimation of proteins?

А. Biuret reaction;

B. Xanthoprotein test;

C. Ninhydrin reaction;

D. Reaction with copper sulfate;

E. Reaction with ammonium sulfate.

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