The rhesus system was discovered in 1937-1940. K.Landsteiner and V.Viner.

The rhesus system was discovered in 1937-1940. K.Landsteiner and V.Viner.

Antigens are found in red blood cells. The most important are D, C, E. However, 15% of Europeans do not, such people are called Rh-negative. If such a person is transfused with Rh-αpositive blood, then there will be a rhesus-conflict.

Practical work 2. Determination of Rh factor by means of serum anti - D.

For this work you need:anti-D, dish, scarifier, alcohol, iodine, cotton wool, glass object.

Technique:on a plate put a drop of anti-D serum, with the help of a slide make a drop of blood, stirred, after 2 minutes. Read the result.

Result: Specify the presence of agglutination, sketch the result.

Conclusion: to determine the presence of rhesus-factor in the blood of the subject, to explain the mechanism of agglutination.

The rules of blood transfusion:

Only one-group blood is poured

1) determine the blood group of the donor and recipient

2) determine the Rh factor

3) conduct a test for physiological compatibility

4) conduct a test for biological compatibility

Practical work 3 . Determination of bleeding time Duke’s method.

For this work you need:scarifier, alcohol, cotton wool, filter paper.

Technique: the finger is treated with alcohol, pierced it by scarifier and every 0.5 minutes a drop of blood protruding from the puncture is soaked by the filter paper until the bleeding stops, the norm is 2 to 4 minutes.

Results. Count the number of prints of drops on the filter paper, divide by 2 - this is the minutes.

Conclusions. Compare the result with the normal value. What mechanisms ensure the stopping of bleeding?

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