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Evidence of protein nature of alpha-amylase:



  Evidence of protein nature of alpha-amylase:

1. Thermal denaturation

  Sample Control
Alpha-amylase 0, 5 ml 0, 5 ml
Heating (100oC/5 min) Yes No
Starch 1% 0, 5 ml 0, 5 ml
Incubation

37oC 10 min
Lugol's solution 3 drops 3 drops
Colour blue yellow

 

2. Proteolysis.

  Sample Control
Alpha-amylase 0, 5 ml 0, 5 ml
Pepsin solution 0, 5 ml -
0, 9% NaCl - 0, 5 ml
Incubation

37oC 10 min
Starch 0, 5 ml 0, 5 ml
Incubation

37oC 10 min
Lugol's solution 3 drops 3 drops
Colour blue yellow

 

Influence of pH on activity of alpha-amylase

Components of reaction

pH of buffer solution
6, 0 6, 4 6, 8 7, 2 7, 6 8, 0
Alpha-amylase 0, 5 ml 0, 5 ml 0, 5 ml 0, 5 ml 0, 5 ml 0, 5 ml
Buffer solution with correspondent pH 2, 0 ml 2, 0 ml 2, 0 ml 2, 0 ml 2, 0 ml 2, 0 ml
Starch solution 0, 5 ml 0, 5 ml 0, 5 ml 0, 5 ml 0, 5 ml 0, 5 ml
Incubation

37oC 10 min
Lugol's solution 3 drops 3 drops 3 drops 3 drops 3 drops 3 drops
Colour blue pink yellow yellow pink blue

 



  

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