Mechanism of anti-viral immune response.

CLASS 10

Topic: Immune response against viruses. Characteristic features of immune response against protozoa and helminthes. Diagnostic serological reactions: Hemagglutination inhibition (HI) test, Neutralizing (Nt) test.

Questions to be discussed:

1. Mechanism of anti-viral immune response.

2. Characteristic features of anti-protozoal immunity.

3. Characteristic features of anti-helminthes immunity.

2. Serological reactions used for virus identification and anti-viral immunity evaluation (Nt test, HI test).

Practical tasks:

1. Fill in a table of protective mechanisms of anti- bacterial and anti-fungal immunity.

Type of microbe/ surface antigens	Innate immunity factors	Acquired immunity factors	Mechanisms of immune escape
Viruses
Protozoa
Helminthes

2. Explain mechanisms of toxin and virus neutralization tests:

3. Explain mechanism of Hemagglutination inhibition test:

Teacher’s signature __________________

Mechanism of anti-viral immune response.

Immune response against protozoa and helminthes

Date__________________

CLASS 11

Topic: Immunological reactions with labeled reagents (RIA, IF, ELISA, Western Blot), their application. Immunity status of the human body.

Questions to be discussed:

1. Immunological reactions with labeled reagents, their advantages.

2. Immunofluorescence tests, advantages and disadvantages of direct and indirect tests.

3. Enzyme immunoassays (EIA, ELISA), types of ELISA (sandwich, capture, competitive).

4. Immunoblotting techniques (Western Blot).

5. 1. Immunity status of the body, definition, stages of evaluation.

6. 2. 1^st and 2^nd level tests of the immunity status evaluation.

Practical task:

1. Draw charts of direct, indirect, and competitive ELISA, using the following symbols:

u - antigen;

Y - specific antibody;

¥ - anti-immunoglobulin (secondary antibody);

E - enzyme label.

☼ -fluorescent dye

X- isotope

1. Draw a chart of the direct and indirect immunofluorescent and Elisa tests.

Direct IFA

Indirect IFA

Direct “sandwich” ELISA

Competitive ELISA

Draw a chart of the Western blot for patient’s anti-HIV antibody, used as confirmation test for diagnosis of HIV-infection - Fig. 3.

gp 160

Blotting

onto NC

paper

Incubation

with patient’s serum

Incubation

with secondary

Ab labeled with enzyme and conversion of the substrate

gp 120

р 61

gp 41

р 24

р 17/18

р 7/9

Fig. 1. Western blot. A, distribution of the HIV antigens (Ags) separated by gel electrophoresis.

B, Ags blotted onto nitrocellulose paper. C, Strip of NC paper treated with patient’s serum. D, Strip of NC paper treated with conjugated anti-human serum. Enzyme conversion of the substrate identifies the presence of antibody to several HIV proteins.

Conclusion: _________________________________________________________

____________________________________________________________________.

3. Explain the mechanism of free-flow cytofluorimetry, used for immune status evaluation.

4. Fill in a table with the tests used for immunity status evaluation.

Immune status

tests

1^st level tests

2nd level tests