Description. Schematic. Table of features

11889

Description

Messenger RNA encoding the full-length SARS-CoV-2 spike glycoprotein.

Schematic

UTR = Untranslated region; sig = extended signal sequence of the S glycoprotein; S protein_mut = S glycoprotein sequence containing mutations K986P and V987P; poly(A) = polyadenylate signal tail.

5‘- capping structure

cap G¹A² = m⁷G⁺m^3'-5'-ppp-5'-Am^2'-3'-p-
[m⁷ = 7-CH₃; m^3' = 3'-O-CH₃; m^2' = 2'-O-CH₃;
-ppp- = -PO₂H-O-PO₂H-O-PO₂H)-; -p- = -PO₂H-]

m¹Ψ = 1-methyl-3'-pseudouridylyl

Table of features

Element	Description	Position
cap	A modified 5’-cap1 structure (m⁷G⁺m^3'-5'-ppp-5'-Am)	1-2
5’-UTR	5´ -untranslated region derived from human alpha-globin RNA with an optimized Kozak sequence	3-54
sig	S glycoprotein signal peptide (extended leader sequence), which guides translocation of the nascent polypeptide chain into the endoplasmic reticulum.	55-102
S protein_mut	Codon-optimized sequence encoding full-length SARS-CoV-2 spike (S) glycoprotein containing mutations K986P and V987P to ensure the S glycoprotein remains in an antigenically optimal pre-fusion conformation; stop codons: 3874-3879 (underlined)	103-3879
3’-UTR	The 3´ untranslated region comprises two sequence elements derived from the amino-terminal enhancer of split (AES) mRNA and the mitochondrial encoded 12S ribosomal RNA to confer RNA stability and high total protein expression.	3880-4174
poly(A)	A 110-nucleotide poly(A)-tail consisting of a stretch of 30 adenosine residues, followed by a 10-nucleotide linker sequence and another 70 adenosine residues.	4175-4284

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