Department of Biochemistry. The theme of the lesson № 7: Biological oxidation. The respiratory chain. Structure and functions of dehydrogenases. Oxidative phosphorylation. Microsomal cytochrome P450 monooxygenase system.

Medical Academy named after S. I. Georgievsky of Vernadsky CFU

Department of Biochemistry

Semester 3. The general ways of metabolism. Metabolism of carbohydrates, lipids and its regulation

The theme of the lesson № 7: Biological oxidation. The respiratory chain. Structure and functions of dehydrogenases. Oxidative phosphorylation. Microsomal cytochrome P450 monooxygenase system.

The purposes of the lesson are:

1) to understand the specificity of biological oxidation and oxidative phosphorylation.

2) to study the main aspects of microsomal oxidation;

Recommendations for preparation of the lesson:

It's necessary to know:

1) the modern interpretation of biological oxidation process;

2) structure of the main enzymes, which take part in biological oxidation;

3) the main scheme of biological oxidation;

4) the location of respiratory chain enzymes on the inner mitochondrial membrane;

5) the connection of oxidative phosphorylation process with mitochondrial electron transport chain.

6) the characterictic of microsomes;

7) oxygenases may be divided into two subgroups: monooxygenases and dioxygenases. Characterise them;

8) Microsomal cytochrome P₄₅₀ monooxigenase systems take part of biosyntheses of steroid hormones from cholesterol and are important for hydroxylation of many drugs;

Review questions:

1. Write the full respiratory chain and name all the enzymes.

2. Write the formula of NAD. Show the reduction process of it.

3. Write the formula of FAD. Show the reduction process of it.

4. Write the formula of CoQ and show the reduction process of it.

5. Tell about cytochromes (their structure and biological properties).

6. Oxidative phosphorylation. Mitchell¢s theory (chemiosmotic theory).

7. Net charge of the respiratory process.

8. Characterize the microsomal fraction.

9. Main enzymes of microsomal oxidation and their role.

References:

1. Robert K. Murray, Daryl K. Granner, Peter A. Mayes, Victor W. Roduell. Harper's Biochemistry (25-th edition), 2000. − P. 8, 130−136.

2. Michael Lieberman, Allan D. Marks. Basic Medical Biochemistry A clinical approach (4-th edition), 2013. – P. 336, 377 – 396.

3.http://103.4.234.46/books/Lippincotts%20Illustrated%20Reviews%20Biochemistry%205th%20edition.pdf

4. Summary