Individual work. student of _____ group. Lesson 4. Enzymes: Structure and properties. CLASSIFICATION OF ENZYMES.

Individual work

student of _____ group

_______________________________________________

^(name)

Lesson 4. Enzymes: Structure and properties. CLASSIFICATION OF ENZYMES.

1. Describe these terms and concepts:

Denaturation-_______________________________________________________________

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active site-__________________________________________________________________

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2. Match what enzyme’s site what function has (show by arrows):

sites of enzyme molecule:	Functions:
1. active	Place of effector binding
2. allosteric	Place of antibody binding
3. montage	Place of substrate binding
4. antigen	Place of subunits binding (for enzymes, which have quaternary structure) or binding with membrane

3. Mark different sites of enzyme molecule on this scheme: (Е-effector, S-substrate, С - coenzyme).

1-_________________________________________________________________

2-_________________________________________________________________

3-_________________________________________________________________

4-_________________________________________________________________

4. Fill in scheme of complex enzyme molecule composition:

5. What substances take part in active site of complex enzymes formation?

А. inhibitors

B. substrate

C. coenzyme

D. membrane’s components

6. Fill in scheme:

7. Draw the graphs of optimal enzymes activity which is influenced by pH of medium:

а) pepsin

б) α-amylase

в) alkaline phosphatase.

activity

1 2 3 4 5 6 7 8 9 10 11 12 13 14 рН

8. Write modern international classification of enzymes:

9. What is a base of modern international classification of enzymes?

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10. What sense of enzyme’s code, for example 1.1.1.27; 2.6.1.5. etc.?

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11. Mark what enzyme’s classes catalyzes type what of the reaction (by numbers).


1. oxidoreductases	catalyze synthetic reactions
2. ligases	catalyze reactions of isomerisation
3. isomerases	catalyze reactions of cleavage of molecules with the partition water molecules
4. lyases	catalyze oxidative-reductive reactions
5. transferases	catalyze reactions of transferring different functional groups
6. hydrolases	catalyze synthetic reactions of cleavage or synthesis of molecules with out partition of water molecules

12. Choose from the enzymes written below the oxidoreductases (underline them)

alcohol dehydrogenase, urease, creatine kinase, lactate dehydrogenase, alanine aminotransferase, glucokinase, acetyl choline esterase, phosphatase, pyruvate decarboxylase, phosphotriose isomerase, glutamate dehydrogenase .